The E2F family of transcription factors has been intensely studied since the discovery that E2F1 can interact with the retinoblastoma tumor suppressor protein. Candidate gene approaches have led to the identification of E2F targets that play a role in cell cycle regulation, DNA replication,...

The functional genomics techniques for mapping transcription regulatory networks have evolved based on advances in experimental approaches and the kinds of data generated. Studies in yeast have emphasized powerful genetic approaches that are not available in other higher eukaryotic organisms....

The development of DNA microarrays has provided considerable impetus to research on genetic regulatory networks since it has enabled genome-wide snapshots of the cellular response to physiological changes. This has led to a revolution in the speed and scope of genetic analysis of regulatory...

The completion of genome sequencing in various organisms, together with the matura- tion of novel high-throughput biotechnologies and the development of powerful com- putational algorithms now allow the application of systems-level analysis to biological networks. Notable achievements of...

Nuclear Factor-kB (NF-kB) is a cytoplasmic transcription factor that plays a key role in inducible gene expression during activation of the innate immune response. Cell stimulation with bacterial endotoxins, viral replication, or by cytokine cascades activates signaling pathways to...

Microarray-based technologies have enabled comprehensive transcriptome profiling. It is becoming feasible to reconstruct gene transcriptional regulatory networks from microarray data. In this chapter, I outline a new strategy for reconstructing gene regulatory networks as part of the causal...

Recent advances in molecular biology and microarray technology have opened the door for massively parallel analysis of transcriptional networks. The development of chromatin immunoprecipitation methods allows for the assessment of transcription factor to target binding in vivo, and powerful...

The cells in the adult liver retain the capacity to proliferate in response to a loss of liver mass. The partial hepatectomy model in rodents has been extensively used to investigate the mechanisms responsible for hepatic growth and proliferation and is the best in vivo model of synchronous...

Cell functioning is dictated by gene expression programs involving regulated transcription of thousands of genes. Gene expression programs result from the binding of transcriptional regulatory proteins to specific promoter sequences. A transcriptional regulatory network can be described as the...

The ability to analyse the expression of thousands of genes in one experiment is revolutionizing the study of gene transcription. Many microarray platforms now exist that allow the determination of transcript expression levels for entire genomes such as yeast and for at least all of the annotated...